In fluorescence imaging, signal intensity is dependant on many factors. Besides tissue specific characteristics and pathology, imaging parameters play a significant role.
These include:
distance, viewing angle, ambient light, dye emission spectrum, camera system sensitivity, attached optics (i.e. scope), system settings, exposure time, digital gain.
Concentration Standards
By imaging tissue samples with a fixed standard (i.e. Absofi reference card), fluorescent images are calibrated according to a standardised benchmark, creating a true reference factor. In essence, it’s like imaging a centimeter scale!
Our standard ICG reference cards have a log concentration scale from 0.03 up to 10 micro molar. These values cover typical bandwidth of clinically relevant values
– In conjunction with the reference cards, the online web application allows for uploading tissue images – The software recognises the reference charts and fits a curve according to concentration values – Tissue samples are then annotated with corresponding values. – Sliders allow for thresholding, contour detection and colour mapping – Export to standard formats (I.e. jpeg) – The portal is camera agnostic, accepting all images